Analysis of chromosome pairing and recombination

We analyzed chromosome pairing in allohexaploid F. arundinacea, its progenitors F. pratensis and F. glaucescens, and intergeneric hybrids L. multiflorum (2x) × F. arundinacea (6x) and L. multiflorum (4x) × F. glaucescens (4x). To evaluate pairing of individual chromosomes, we analyzed tetraploid lines of L. multiflorum substitution from F. pratensis. GISH permitted the analysis of homoeologous chromosome pairing and recombination of different genomes involved.

Kopecký, D., Havránková, M., Loureiro, J., Castro, S., Lukaszewski, A.J., Bartoš, J., Kopecká, J. and Doležel, J. (2010). Physical distribution of homoeologous recombination in individual chromosomes of Lolium multiflorum/Festuca pratensis substitutions. Cytogenetic and Genome Research 129:162-172.

Kopecký, D., Bartoš, J., Zwierzykowski, Z. and Doležel, J. (2009). Chromosome pairing of individual genomes in tall fescue (Festuca arundinacea Schreb.), its progenitors, and hybrids with Italian ryegrass (Lolium multiflorum Lam.). Cytogenetics and Genome Research 124: 170-178.

Kopecký, D., Lukaszewski, A.J. and Doležel, J. (2008). Meiotic behavior of individual chromosomes of Festuca pratensis in tetraploid Lolium multiflorum. Chromosome Research 16: 987-998.