Dark matter in plant cell nuclei – characterization of nuclear proteins.

Start date: 
Wednesday, January 1, 2014
End date: 
Saturday, December 31, 2016
Most of genetic information in eukaryotes is stored in cell nuclei, which are highly organized and dynamic organelles. Various proteins were described to play essential role in packing DNA, its replication and transcription in the nuclei of human and some model species. However, there is almost no information about the majority of these proteins in plants. This reminds dark matter in the universe, whose existence is generally accepted, but its nature remains obscure. In order to reveal the secrets of this component of plant cell nuclei, and to discover as yet uncharacterized plant nuclear proteins and their possible interactors, we will employ multidisciplinary approaches, including proteomics, flow cytometry and immunohistochemistry. In addition to identifying new proteins, we will perform localization analysis of some of them and follow changes in nuclear protein composition during cell cycle. The results will shed light on the nature and function of the vital component of plant cell nuclei.
Grant agency: 
Registration number: 
Internal investigator/coinvestigator: 
Beáta Petrovská
Internal investigator: 
is coinvestigator only
External investigator/coinvestigator: 
Univerzita Palackého v Olomouci