Flow cytometric chromosome sorting in plants: The next generation

Vrána, J., Šimková, H., Kubaláková, M., Číhalíková, J., Doležel, J.
METHODS 57: 331-337, 2012

Keywords: Chromosome sorting, Flow cytometry, Fluorescence in situ hybridization, Next generation sequencing, Whole genome amplification
Abstract: Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.
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IEB authors: Jarmila Číhalíková, Jaroslav Doležel, Marie Kubaláková, Hana Šimková, Jan Vrána