Physical map of wheat chromosome arm 7DS and its use to clone a Russian wheat aphid resistance gene

Start date: 
Sunday, January 1, 2012
End date: 
Thursday, December 31, 2015
Map-based cloning in bread wheat is a daunting task due to enormous size (~17 Gbp) and polyploid nature of its genome. One of the strategies facilitating accomplishment of this goal is use of genomic resources created from smaller segments of the genome - chromosomes or chromosome arms. Here we propose assembling clones of a BAC library previously constructed from the short arm of wheat 7D chromosome into a physical map. The ordered clones will be sequenced by Illumina technology and assembled. The resulting BAC clone sequences will enable landing of the physical map on the chromosome. The integrated map as well as clone-by-clone sequence of the 7DS will signicantly speed up cloning of all genes from this part of the wheat genome. Within the project, we assume map-based cloning of a gene conferring resistance to one of the most devastating wheat pests, Russian wheat aphid (Diuraphis noxia) located on 7DS. Besides of elucidating molecular basis of the trait, the knowledge of the gene enables host-pathogen interaction studies and development of markers for wheat breeding.
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Internal investigator/coinvestigator: 
Hana Šimková
Internal investigator: 
is principal investigator