SSR genotyping

The genotyping systems is based on 19 microsatellite loci, that are scored using fluorescently labeled primers and high-throughput capillary electrophoresis separation with high resolution. This genotyping platform was tested and optimized on a set of 70 diploid and 38 triploid banana accessions and nowadays is used to characterize Musa accessions in the ITC collection in cooperation with Bioversity International.

The marker set used in this study provided enough polymorphism to discriminate between individual species, subspecies and subgroups of all accessions of Musa. Likewise, the capability of identifying duplicate samples was confirmed. Based on the results of a blind test, the genotyping system was confirmed to be suitable for characterization of unknown accessions.

For detailed information, please see: Pavla Christelová, Miroslav Valárik, Eva Hřibová, Innes Van den houwe, Stephanie Chanelliere, Nicolas Roux, Jaroslav Doležel: A platform for efficient genotyping in Musa using microsatellite markers. AoB Plants 2011. doi: 10.1093/aobpla/plr024.