Use of single gene FISH for analyzing homoeologous relationships between wheat and Crested wheatgrass

Said M., Danilova T., Friebe B., Gill B.S., Doležel J.
Research in plant genetics (From Mendel's peas to the present) Conference | 7 – 10 September | Brno - Czech Republic : , 2015

Keywords: Agropyron cristatum, single gene fluorescence in situ hybridization, FISH, homoeologous relationships
Abstract: Agropyron cristatum L. (Gaertn.) commonly known as Crested Wheatgrass is a wild species and may provide novel genes for wheat improvement. The genomes of allohexaploid bread wheat and other species from Triticeae are colinear to some extent, i.e., composed of homoeoloci at similar positions along the chromosomes, and with genic regions being highly conserved. Fluorescence in situ hybridization (FISH) facilitates construction of chromosome physical maps for studying evolutionary rearrangements. Mapping of single-copy sequences such as full length complementary DNA (FLcDNA) is a highly effective and attractive approach. For analyzing the structure and homoeology of A. cristatum chromosomes a set of wheat FLcDNA probes; seven from group-3 and nine from group-5 was applied on two wheat-A. cristatum addition lines. The 5P chromosome addition line has a pair of 5P chromosomes added to the hexaploid wheat chromosome complement, whereas the 3P addition line has one complete 3P and one 3PS telosome. Each probe was also applied to diploid cv. Parkway of A. cristatum (2n=2x=14, PP). All wheat probes were successfully detected on the A. cristatum chromosomes. The FLcDNA probes from wheat group-3 were physically mapped on 3P, three on the short arm and four on the long arm. The probes from group-5 were physically mapped on 5P, five on the short arm and four on the long arm. No major rearrangements were detected in chromosomes 3P and 5P added to wheat. Work is in progress to determine the homoeologous relationships of the remaining A. cristatum chromosomes.
IEB authors: Jaroslav Doležel, Mahmoud Said