Will orchids reshape our understanding of genome-wide processes? Solving the enigma of progressively partial endoreduplication

Start date: 
Sunday, January 1, 2012
End date: 
Thursday, December 31, 2015
The project aims at investigating the phenomenon of progressively partial endoreduplication (PPE) that has recently been discovered in the genome of some orchids. Whereas both the generative and somatic polyploidy have been intensively researched, nothing is known about the patterns and processes behind the PPE. With the aid of advanced molecular cytogenetic techniques (flow cytometry and sorting, Illumina next-generation sequencing, FISH), we will address the incidence of PPE across the family Orchidaceae and search for correlations with phylogeny and species traits. Ludisia discolor, a taxon with a small genome size and ca 50% PPE, will serve as a model system to elucidate differences in sequence composition between normal (2C) and partially reduplicated genomes. To localize structural changes in the genome and assess their phylogenetic stability, repetitive elements showing different quantities will be mapped to interphase nuclei and somatic chromosomes of Ludisia and related species. The results will provide a new level of understanding of genome-wide processes in eukaryotes.
Grant agency: 
Registration number: 
Internal investigator/coinvestigator: 
Jan Vrána
Internal investigator: 
is coinvestigator only
External investigator/coinvestigator: 
Suda Jan, Čurn Vladislav