Institute of Experimental
Botany of the AS CR, v.v.i.
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Flow cytometry
Reference DNA standards
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Contact
Staff
Mahmoud Said
Mahmoud Said Ph.D.
Position
Post doc
Phone
+420 585 238 718
E-mail
said@ueb.cas.cz
Address
Šlechtitelů 31, 779 00 Olomouc
Room number
302
Research group
Cápal Group
Curriculum Vitae
Education and Academic Degrees
2005–2010 –
Ph.D.
, Genetics, plant breeding, cytogenetics and molecular genetics, University of Cordoba, Spain
2004–2007 –
M.Sc.
,Genetics, plant breeding, molecular genetics and cytogenetics CIHEAM – IAMZ, Spain
2004–2006 –
Diploma
of Advanced Studies in Genetics, University of Cordoba, Spain
2002–2003 –
Diploma
of post-graduation Specialization Studies in Plant breeding, CIHEAM - IAMZ, Spain
1995–1998 –
M.Sc.
, Agronomy, Alexandria University, Egypt
1988–1992 –
B.Sc.
, Agricultural Science, University of Assiut, Egypt
Position and Employment
2013–present –
Postdoctoral Fellow
, Laboratory of Molecular Cytogenetics and Cytometry, Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany, Czech Academy of Sciences, Olomouc, Czech Republic
2014–2015 –
Postdoctoral Fellow
, Centre of the Region Hana for Agricultural and Biotechnological Research, Olomouc, Czech Republic
2011–2012 –
Postdoctoral Research Fellow
, Department of Genetics, University of Cordoba, Cordoba, Spain
2005–2010 – Research project for
Ph.D. program
, Department of Genetics, University of Cordoba, Cordoba, Spain
1994–2004 –
Associate Researcher
at Agricultural Research Center, Ministry of Agriculture, Egypt
Research Focus
Plant genome structure, molecular markers, molecular biology, DNA-cloning and sequencing, chromosome physical mapping, genome comparisons and evolution
Plant breeding, interspecific hybridization, alien chromosome introgressions, genetic diversity, wild relatives, germplasm resources, chromosome deletions and translocations development
Cytogenetics,
in situ
hybridization FISH and GISH, physical localization of single-gene and low-copy sequences, cDNA probes, genome karyotyping and chromosome identification, optical and fluorescence microscopy, image processing, cytological analysis
Flow Cytometry, chromosome flow sorting, genome dissection into individual chromosomes, DNA content, genome size estimation
Experimental design and statistical methods, morphological and agronomic characterization, fungal disease evaluation, data handling, field trials implementation and management
Publication Activity
Publications in peer-reviewed journals: 8
Number of citations: 77
h
-index: 5
(Web of Science, January 2020)
Five Selected Publications
Said, M.
, Kubaláková, M., Karafiátová, M., Molnár, I., Doležel, J., Vrána, J. (2019) Dissecting the complex genome of crested wheatgrass by chromosome flow sorting. The Plant Genome. 12: 180096
Said, M.
, Parada, A. C., Gaál, E., Molnár, I., Cabrera, A., Doležel, J., Vrána, J. (2019) Uncovering homeologous relationships between tetraploid
Agropyron cristatum
and bread wheat genomes using COS markers. Theoretical and Applied Genetics. 132: 2881–2898
Said, M.
, Hřibová, E., Danilova, T., Karafiátová, M., Čížková, J., Friebe, B., Doležel, J., Gill, B. S., Vrána, J. (2018) The
Agropyron cristatum
karyotype, chromosome structure and cross-genome homoeology as revealed by Fluorescence
in situ
Hybridization with tandem repeats and wheat single-gene probes. Theoretical and Applied Genetics 131: 2213–2227
Ochoa, V., Madrid, E.,
Said, M.
, Rubiales, D., Cabrera, A. (2015) Molecular and cytogenetic characterization of a common wheat-
Agropyron cristatum
chromosome translocation conferring resistance to leaf rust. Euphytica 201: 89– 95
Said, M.
, Recio, R., Cabrera, A. (2012) Development and characterization of structural changes in chromosome 3H
ch
from
Hordeum chilense
in common wheat and their use in physical mapping. Euphytica 188: 429–440
Publications