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Annotation: „DNA-protein crosslinks, i.e. proteins covalently attached to DNA molecule represent extremely toxic type of DNA damage, which hinder the essential processes of replication and transcription. Here, we set-up forward-directed genetic screen to identify mutants hypersensitive to DNA protein crosslink inducing chemical zebularine that crosslinks METHYLTRANSFERASE1 (MET1) at 45S rDNA repeats. We mapped HYPERSENSITIVE TO ZEBULARINE 1 (HZE1 . . .

Annotation: Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. In this work we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues. We found that dividing nuclei have a round shape, adherent sister chromatids keep an equal amount of CENH3 histone and organize chromosomes according to a . . .

Annotation:  Mitosis and cytokinesis are fundamental processes through which somatic cells increase their numbers and allow plant growth and development. Here, we analyzed the organization and dynamics of mitotic chromosomes, nucleoli, and microtubules in living cells of barley root primary meristems using a series of newly developed stable fluorescent protein translational fusion lines and time-lapse confocal microscopy. The median duration of . . .

Annotation: Early cytological studies indicated that chromatids of mitotic chromosomes coil into a helix but a direct proof for this assumption was missing. Here, we combined Hi-C analysis of purified metaphase chromosomes, biopolymer modelling and spatial structured illumination microscopy to reveal a helically-wound, 400 nm thick chromatin thread forming barley mitotic chromatids. Chromatin from adjacent turns of the helix intermingled due to the . . .

Annotation: The last decade has seen a proliferation in genomic resources for wheat, including reference- and pan-genome assemblies with gene annotations, which provide new opportunities to detect, characterise, and describe genes that influence traits of interest. To accommodate these developments, an updated set of community-agreed guidelines for gene nomenclature in wheat has been compiled, enabling to describe loci based on morphological or . . .

Annotation:  RUVBL proteins are evolutionary conserved helicases which absence leads to complete lethality of the progeny. In this work we analyzed the role of RUVBLs in plant gametophyte development that could be the reason for absence of homozygous progeny.  ruvbl mutants have strongly impacted female gametogenesis producing an aberrant embryo sac. Additionally, male gametogenesis was compromised by reduced pollen germination and pollen tube . . .

Annotation: Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity1. However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value2. Faba bean (Vicia faba L.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here . . .

Annotation:  Occurrence of Festuca apennina De Not. (4 × Fape), F. pratensis Huds. (2 × Fp) and the triploid, sterile hybrid F. apennina × F. pratensis (3 × Fape × Fp) was studied in 12 regions of the Swiss Alps. Triploid hybrid was more frequent and more dominant than both parental species around 1400 m, and . . .

Annotation: Some interspecific plant hybrids show unequal transmission of chromosomes from parental genomes to the successive generations. We revealed that this is caused by the differential behavior of parental chromosomes during meiosis. In male meiosis of Festuca x Lolium hybrids, Festuca univalents were attached less frequently to microtubules than Lolium univalents, lagged in divisions and formed micronuclei, which were subsequently eliminated . . .

Annotation: Vernalization is a period of low non-freezing temperatures, which provides the competence to flower. This mechanism ensures that plants sown before winter develop reproductive organs in more favourable conditions during spring. Such an evolutionary mechanism has evolved in both monocot and eudicot plants. Studies in monocots, represented by temperate cereals like wheat and barley, have identified and proposed the VERNALIZATION1 (VRN1) gene as . . .

Annotation: Wild emmer wheat is an excellent reservoir of genetic variability that can be utilized to improve cultivated wheat to address the challenges of the expanding world population and climate change. Bearing this in mind, we have collected a panel of 263 wild emmer wheat (WEW) genotypes across the Fertile Crescent. The genotypes were grown in different locations and phenotyped for heading date. Genome-wide association mapping (GWAS) was carried . . .

Annotation:  Breeding of wheat adapted to new climatic conditions and resistant to diseases and pests is hindered by a limited gene pool due to domestication and thousands of years of human selection. Annual goatgrasses (Aegilops spp.) with M and U genomes are potential sources of the missing genes and alleles. Development of alien introgression lines of wheat may be facilitated by the knowledge of DNA sequences of Aegilops chromosomes. As the . . .

Annotation: Sequence assemblies of most plant genomes remain fragmented, leaving the aim of gapless, telomere-to-telomere (T2T) sequence assemblies unattained. Revealing the causes of sequence gaps in the current assemblies helps determine the resources needed to close them. We analysed sequence gaps in the current long-read reference genome sequence of barley cv. Morex (MorexV3) by the optical map and sequence raw . . .

Annotation: While most eukaryotes contain single regional centromeres, several plant and animal lineages assemble holocentromeres along the entire chromosome length. In our study, we assembled genomes of three beak sedges (Rhynchospora pubera, R. breviuscula, and R. tenuis) with repeat-based holocentromeres as well as a genome of their closest monocentric relative, Juncus effusus, and compared their genome organization and evolution as a function of . . .

Annotation: 45S ribosomal DNA loci are organized as long head-to-tail tandem arrays of nearly identical units, spanning several megabases of sequence. Due to this structure, the rDNA loci are the major sources of gaps in genome assemblies, and expression status of particular arrays remains elusive. We combined cytogenomics techniques with bioinformatics to reconstruct individual 45S rDNA arrays in hexaploid wheat, thus building a stepping stone for . . .