News

New publication: Deregulated Phosphorylation of CENH3 . . .
Annotation: Mutants in centromeric histone variant, cenh3-1, complemented by CENH3 S65A or S65D are defective with respect to their floral meristem development. (A) Main stems of Wt and cenh3-1 complemented mutants (S65S, S65A or S65D under native promotor). (B) Scanning electron micrograph of flowers of Wt and cenh3-1 complemented mutants (S65S, S65A or S65D under native promotor).Link
New publication: Nuclear Disposition of Alien . . .
Annotation:  Using 3D FISH, we studied alien chromosome posiotining in various wheat-rye and wheat-barley introgression lines. In the majority of cases, the detected homologous chromosomes did not show any association. Interestingly, there appeared to be a relationship between the chromosome length and its positioning in the nucleus: longer chromosomes frequently occupied position close to the nuclear periphery, while shorter chromosomes were . . .
Czech scientists are joining the call of European . . .
A year ago, the European Court of Justice (ECJ) ruled that plants obtained by methods of precision breeding (so-called CRISPR) are genetically modified organisms. However, this means that even crops with the smallest genome modifications that can occur spontaneously in nature are subject to restrictive European regulations prohibiting such modifications. 
New publication: Chemically-induced DNA de-methylation . . .
Annotation: Effect of 5-azacytidine pre-treatment on microspore reprogramming and the effectiveness on microspore embryogenesis in triticale. Top panel: Morphology of microspores on the day of in vitro cultures initiation. Bars = 50 µm. Middle panel: Embryo-like structures (yellow arrows) formed after six weeks of in vitro cultivation. Bars = 2 mm. Bottom panel: Green and albino (white arrows) plants regenerated after nine weeks from microspores . . .
New publication: The SMC5/6 complex subunit NSE4A is . . .
Annotation:  Confocal imaging of live seed of Arabidopsis 72 hours after pollination. Red color shows cell walls and yellow NSE4A protein in cell nuclei of the syncytial (multi-nucleate) endosperm. Bar = 50 µm.Link
IWGSC writes about Jaroslav Doležel
Jaroslav Doležel is Head of the Centre of Plant Structural and Functional Genomics of the Institute of Experimental Botany (IEB) and Professor of Molecular Biology and Genetics at Palacký University with both institutes in Olomouc (Czech Republic).
New publication: Dissecting the complex genome of . . .
Annotation:  Flow sorting enabled to dissect the genome of crested wheatgrass into individual chromosomes. The flow sorted chromosome- or chromosome-arm-specific subgenomic DNA samples provide attractive resources to perform structural and functional genome analysis. Successful sorting of B chromosomes (Bs) of crested wheatgrass provides an opportunity to determine their gene content and reveal their origin, as well as discovering the mechanisms . . .
New publication: Selective Elimination of Parental . . .
Annotation:  Alien introgressions are often used to introduce beneficial traits from one species into another. However, exploitation of the introgressions in breeding requires proper transmission of introgressed segments to consecutive generations. In introgression cultivars of xFestulolium hybrids, we observed about 27–32% elimination of Festuca chromatin in a single round of multiplication. At this pace, Festuca chromatin would be . . .
New publication: The Dark Matter of Large Cereal . . .
Annotation: Recently published reference genomes of important small grain cereals were shown to represent 88 – 98% of their genome size. We aimed at identifying the missing parts in genomes of wheat and barley, considering tandem repeats as the major candidates. We found that shorter repeats can be present in the genome assemblies even if generated from short-read data but they may be difficult to integrate into pseudomolecules. We demonstrated . . .
Olomouc Research into the Impact of Climate Change on . . .
Pranav Sahu, a scientist from the Olomouc workplace of the Institute of Experimental Botany of the Czech Academy of Sciences was awarded in India for his research into plants and their reactions to climate change. His work was awarded the second place at a symposium of the European Molecular Biology Organization, taking place on 15-17 April 2019 in Delhi.  
New publication: Divergence between bread wheat and . . .
Annotation: Blumeria graminis causing disease powdery mildew is serious pest of wheat casing significant loses in yield and quality. Introgression of new resistance genes from wild related species is attractive way for wheat genome improvement. However, the introgression is often accompanied with deleterious gene introduction and loss of recombination hampering cloning of such genes. If you are interest in way how to clone such introgressed powdery . . .
New publication: Inter-morphotype hybridization in . . .
Annotation: Homoeologous chromosome pairing provides an opportunity to introgress chromosome segment from one species to another. On the other hand, it may cause destabilization of hybrid genome via formation of multivalents and univlents, and consequntly, the loss of chromosomes and/or uneven segregation. We developed hybrids of Mediterranean and Continental morphotypes of tall fescue (Festuca arundinacea Schreb.) with aim to introgress summer dormancy . . .
New publication: Misdivision of Telocentrics and . . .
Annotation: We analyzed the meiotic behavior of misdivision products of meiosis: isochromosomes and telocentrics in wheat. The 2 most frequent scenarios were separation of sister chromatids in anaphase I or monopolar/bipolar attachment of the univalent to the spindle apparatus with unseparated chromatids. Misdivision was rare, and its frequency appeared directly related to the size of the centromere. Pairing of identical arms in isochromosomes did not . . .
New publication: The Coiled-Coil NLR Rph1, Confers . . .
Annotation: The  tools for unraveling the mechanisms of disease resistance in cereal crops are currently limited due to their large repeat-rich genomes and the lack of genetic recombination or cultivar (cv)-specific sequence information. We cloned the first leaf rust resistance gene Rph1 from cultivated barley using “MutChromSeq,” a recently developed molecular genomics approach for the rapid cloning of genes in plants. Flow-sorted 2H . . .
The Application Laboratory for Agricultural Research . . .
The Application Laboratory for Agricultural Research is the first laboratory at the Czech Academy of Sciences to link scientists with breeders and farmers. It was established under the Food for Future Program of the Strategy AV21 in February 2017 and is located at the Centre of Plant Structural and Functional Genomics of the Institute of Experimental Botany in Olomouc. 
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