News

New publication: Restoring cytonuclear stoichiometry . . .
Annotation: Whole genome duplication and interspecific hybridization are two core processes that underline evolution and speciation in plants. They also represent widely used tools in the plant breeding. Although these two phenomena confer long-term benefits to the plant species, they bring many obstacles to be dealt with in order to stabilize the newly acquired genetic environment. In this study, we examined how newly established polyploids and . . .
New publication: Core promoterome of barley embryo
Annotation:  Precise localization and dissection of gene promoters are key to understanding transcriptional gene regulation and to future bioengineering applications. In this study, we employed cap analysis of gene expression (CAGE) at three embryonic developmental stages in barley to accurately map, annotate, and quantify transcription initiation events. Using novel bioinformatics tools for sequence clustering coupled with annotation of . . .
New publication: SMC5/6 complex-mediated SUMOylation . . .
Annotation: „DNA-protein crosslinks, i.e. proteins covalently attached to DNA molecule represent extremely toxic type of DNA damage, which hinder the essential processes of replication and transcription. Here, we set-up forward-directed genetic screen to identify mutants hypersensitive to DNA protein crosslink inducing chemical zebularine that crosslinks METHYLTRANSFERASE1 (MET1) at 45S rDNA repeats. We mapped HYPERSENSITIVE TO ZEBULARINE 1 (HZE1 . . .
New publication: Non-Rabl chromosome organization in . . .
Annotation: Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. In this work we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues. We found that dividing nuclei have a round shape, adherent sister chromatids keep an equal amount of CENH3 histone and organize chromosomes according to a . . .
New publication: Spatial organization and dynamics of . . .
Annotation:  Mitosis and cytokinesis are fundamental processes through which somatic cells increase their numbers and allow plant growth and development. Here, we analyzed the organization and dynamics of mitotic chromosomes, nucleoli, and microtubules in living cells of barley root primary meristems using a series of newly developed stable fluorescent protein translational fusion lines and time-lapse confocal microscopy. The median duration of . . .
New publication: Helical coiling of metaphase chromatids
Annotation: Early cytological studies indicated that chromatids of mitotic chromosomes coil into a helix but a direct proof for this assumption was missing. Here, we combined Hi-C analysis of purified metaphase chromosomes, biopolymer modelling and spatial structured illumination microscopy to reveal a helically-wound, 400 nm thick chromatin thread forming barley mitotic chromatids. Chromatin from adjacent turns of the helix intermingled due to the . . .
New publication: Updated guidelines for gene . . .
Annotation: The last decade has seen a proliferation in genomic resources for wheat, including reference- and pan-genome assemblies with gene annotations, which provide new opportunities to detect, characterise, and describe genes that influence traits of interest. To accommodate these developments, an updated set of community-agreed guidelines for gene nomenclature in wheat has been compiled, enabling to describe loci based on morphological or . . .
New publication: RUVBL proteins are involved in plant . . .
Annotation:  RUVBL proteins are evolutionary conserved helicases which absence leads to complete lethality of the progeny. In this work we analyzed the role of RUVBLs in plant gametophyte development that could be the reason for absence of homozygous progeny.  ruvbl mutants have strongly impacted female gametogenesis producing an aberrant embryo sac. Additionally, male gametogenesis was compromised by reduced pollen germination and pollen tube . . .
New publication: The giant diploid faba genome unlocks . . .
Annotation: Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity1. However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value2. Faba bean (Vicia faba L.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here . . .
New publication: Festuca apennina × F. pratensis . . .
Annotation:  Occurrence of Festuca apennina De Not. (4 × Fape), F. pratensis Huds. (2 × Fp) and the triploid, sterile hybrid F. apennina × F. pratensis (3 × Fape × Fp) was studied in 12 regions of the Swiss Alps. Triploid hybrid was more frequent and more dominant than both parental species around 1400 m, and . . .
New publication: Both male and female meiosis . . .
Annotation: Some interspecific plant hybrids show unequal transmission of chromosomes from parental genomes to the successive generations. We revealed that this is caused by the differential behavior of parental chromosomes during meiosis. In male meiosis of Festuca x Lolium hybrids, Festuca univalents were attached less frequently to microtubules than Lolium univalents, lagged in divisions and formed micronuclei, which were subsequently eliminated . . .
New publication: Contemplation on wheat vernalization
Annotation: Vernalization is a period of low non-freezing temperatures, which provides the competence to flower. This mechanism ensures that plants sown before winter develop reproductive organs in more favourable conditions during spring. Such an evolutionary mechanism has evolved in both monocot and eudicot plants. Studies in monocots, represented by temperate cereals like wheat and barley, have identified and proposed the VERNALIZATION1 (VRN1) gene as . . .
New publication: Wild emmer wheat, the progenitor of . . .
Annotation: Wild emmer wheat is an excellent reservoir of genetic variability that can be utilized to improve cultivated wheat to address the challenges of the expanding world population and climate change. Bearing this in mind, we have collected a panel of 263 wild emmer wheat (WEW) genotypes across the Fertile Crescent. The genotypes were grown in different locations and phenotyped for heading date. Genome-wide association mapping (GWAS) was carried . . .
New publication: Flow karyotyping of wheat- Aegilops . . .
Annotation:  Breeding of wheat adapted to new climatic conditions and resistant to diseases and pests is hindered by a limited gene pool due to domestication and thousands of years of human selection. Annual goatgrasses (Aegilops spp.) with M and U genomes are potential sources of the missing genes and alleles. Development of alien introgression lines of wheat may be facilitated by the knowledge of DNA sequences of Aegilops chromosomes. As the . . .
New publication: Prospects of telomere-to-telomere . . .
Annotation: Sequence assemblies of most plant genomes remain fragmented, leaving the aim of gapless, telomere-to-telomere (T2T) sequence assemblies unattained. Revealing the causes of sequence gaps in the current assemblies helps determine the resources needed to close them. We analysed sequence gaps in the current long-read reference genome sequence of barley cv. Morex (MorexV3) by the optical map and sequence raw . . .
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