News

New publication: Competition of Parental Genomes in . . .
Annotation:  Our review targets interspecific hybridization, one of the main mechanisms of plant speciation. Genomes of newly established interspecific hybrids undergo dramatic changes including chromosome rearrangements, amplifications of tandem repeats, activations of mobile repetitive elements and gene expression modifications. To ensure genome stability and proper transmission of chromosomes from both parental genomes into subsequent generations . . .
New publication: Development of deletion lines for . . .
Annotation:  The identification of genes of agronomic interest in bread wheat (Triticum aestivum L.) is hampered by its allopolyploid nature and its considerable genome size,  largely covered by transposable elements. In this study, the 2C gametocidal chromosome from Aegilops cylindrica was used to develop a set of 113 deletion lines for chromosome 3D in the reference cultivar 'Chinese Spring'. Eighty-four markers were used to . . .
New publication: Involvement of homocastasterone . . .
Annotation: Effect of cold hardening and drought treatment on the pattern of changes in phytohormone content and their homeostasis in double haploid lines of winter barley extremely differentiated in respect of freezing/drought tolerance level. The summary shows the correlation between the level of phytohormones, stress tolerance and the activity of nonspecific peroxidase (POX) and low molecular weight antioxidants (LMW AOX).Link
New publication: Chromosome painting facilitates . . .
Annotation:  Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. The study demonstrates the potential of chromosome painting for comparative karyotype analysis and identification of structural chromosome changes that accompanied . . .
New publication: CRISPR/Cas9-Based RGEN-ISL allows the . . .
Annotation:  The effects of the non-denaturing RGEN-ISL and standard denaturing FISH on the chromatin structure of maize were compared. A, B Comparison of the chromatin ultrastructure after Knob 2-specific labeling by RGEN-ISL (red) and subsequent FISH (green) of fixed chromosomes (A) and interphase nuclei of maize (B). To analyze the ultrastructure and spatial arrangement of signals and chromatin at a lateral resolution of ∼140 nm, 3D . . .
Universitas: Applied research cannot do without genome . . .
As a little boy he wanted to be a gamekeeper, but eventually his passion for plants won. Plant geneticist Aleš Pečinka from the Center of the Region Haná for Biotechnological and Agricultural Research has moved from classical “flower lover” through cytology to molecular biology and epigenetics.
New publication: Deregulated Phosphorylation of CENH3 . . .
Annotation: Mutants in centromeric histone variant, cenh3-1, complemented by CENH3 S65A or S65D are defective with respect to their floral meristem development. (A) Main stems of Wt and cenh3-1 complemented mutants (S65S, S65A or S65D under native promotor). (B) Scanning electron micrograph of flowers of Wt and cenh3-1 complemented mutants (S65S, S65A or S65D under native promotor).Link
Olomouc scientists help sequence the pea genome
Scientists from the Olomouc laboratory of the Institute of Experimental Botany (IEB) at the Czech Academy of Sciences, which is part of the Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), have achieved another success. They were part of an international team that has sequenced the common pea genome after six years. The findings have been published in the prestigious scientific journal Nature Genetics.
New publication: Nuclear Disposition of Alien . . .
Annotation:  Using 3D FISH, we studied alien chromosome posiotining in various wheat-rye and wheat-barley introgression lines. In the majority of cases, the detected homologous chromosomes did not show any association. Interestingly, there appeared to be a relationship between the chromosome length and its positioning in the nucleus: longer chromosomes frequently occupied position close to the nuclear periphery, while shorter chromosomes were . . .
Czech scientists are joining the call of European . . .
A year ago, the European Court of Justice (ECJ) ruled that plants obtained by methods of precision breeding (so-called CRISPR) are genetically modified organisms. However, this means that even crops with the smallest genome modifications that can occur spontaneously in nature are subject to restrictive European regulations prohibiting such modifications. 
New publication: Chemically-induced DNA de-methylation . . .
Annotation: Effect of 5-azacytidine pre-treatment on microspore reprogramming and the effectiveness on microspore embryogenesis in triticale. Top panel: Morphology of microspores on the day of in vitro cultures initiation. Bars = 50 µm. Middle panel: Embryo-like structures (yellow arrows) formed after six weeks of in vitro cultivation. Bars = 2 mm. Bottom panel: Green and albino (white arrows) plants regenerated after nine weeks from microspores . . .
The function of an important plant gene decoded
Plant geneticists from the Institute of Experimental Botany of the Czech Academy of Sciences (IEB CAS) have been the first in the world to describe the function of two genes of Arabidopsis thaliana and their influence on the properties of this model plant. The prestigious journal Plant Cell has published their work which could have practical applications in the future.
New publication: The SMC5/6 complex subunit NSE4A is . . .
Annotation:  Confocal imaging of live seed of Arabidopsis 72 hours after pollination. Red color shows cell walls and yellow NSE4A protein in cell nuclei of the syncytial (multi-nucleate) endosperm. Bar = 50 µm.Link
IWGSC writes about Jaroslav Doležel
Jaroslav Doležel is Head of the Centre of Plant Structural and Functional Genomics of the Institute of Experimental Botany (IEB) and Professor of Molecular Biology and Genetics at Palacký University with both institutes in Olomouc (Czech Republic).
New publication: Dissecting the complex genome of . . .
Annotation:  Flow sorting enabled to dissect the genome of crested wheatgrass into individual chromosomes. The flow sorted chromosome- or chromosome-arm-specific subgenomic DNA samples provide attractive resources to perform structural and functional genome analysis. Successful sorting of B chromosomes (Bs) of crested wheatgrass provides an opportunity to determine their gene content and reveal their origin, as well as discovering the mechanisms . . .
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