Annotation:
Pm1a, the first powdery mildew resistance gene described in wheat, is part of a complex resistance gene cluster located in a distal region of chromosome arm 7AL that has suppressed genetic recombination, which hampered gene cloning by map-based approach. The paper reports the successful cloning of
Pm1a from common wheat using the method of mutagenesis and resistance (R) gene enrichment sequencing (MutRenSeq), and identification of the corresponding
AvrPm1a effector from the wheat powdery mildew fungus
Bgt using genetic mapping and RNA sequencing (RNAseq). Sequencing of flow-sorted chromosome 7A from cv. Axminster allowed us to isolate sequences from the
Pm1a-bearing chromosome, which provided evidence of rearrangement leading to the suppressed recombination.
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