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Botany of the AS CR, v.v.i.
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Genome dynamics in the context of sex chromosome evolution in the genus Humulus
Genome dynamics in the context of sex chromosome evolution in the genus Humulus
Duration
01/2022 - 12/2024
Agency
Czech Science Foundation
Project number
22-00301S
Principle investigator
RNDr. Roman Hobza, Ph.D.
Co-principle investigator
RNDr. Jan Šafář, Ph.D.
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Sex chromosomes have evolved multiple times in both plants and animals. Numerous studies have shown that distant species may share fundamental properties of sex chromosome formation, while closely related species can differ greatly. We intend to investigate genome dynamics in two closely related dioecious species, Humulus lupulus and H. japonicus. Among the most prominent and interesting differences in these species are the variations in genome size, autosome number and in the sex chromosomes themselves (XX/XY1Y2 in H. japonicus, XX/XY in H. lupulus). Using flow cytometry, sex chromosome sorting and sequencing,
comparative cytogenomics and bioinformatics, we will investigate which elements are major players in genome size variability and sex chromosome formation. Our study will not only provide novel contributions to the understanding of genome dynamics in the studied species but comparison with other sex chromosome possessing plant species will enable us to identify general processes that form and regulate plant genomes.
Project team
Group leader
Jan Šafář Ph.D.
Postdoc
Radim Čegan Ph.D.
Postdoc
Beáta Strejčková Ph.D.