Institute of Experimental
Botany of the AS CR, v.v.i.
Reference DNA standards
Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative
Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies
Tulpová, Z., Lu, M.C., Toegelová, H., Visendi, P., Hayashi, S., Vojta, P., Paux, E., Kilian, A., Abrouk, M., Bartoš, J., Hajdúch, M., Batley, J., Edwards, D., Doležel, D., Šimková, H.
(Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding.
maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of
and to assist
. As a part of a joint effort coordinated by the International
Genome Sequencing Consortium, we have constructed a BAC-based
consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid
and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This
integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up
. The process of
assembly included the integration of the
with a whole-genome
of Aegilops tauschii and a
, which together enabled efficient scaffolding of
contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of
and its ancestor at BAC-contig level and revealed a reconstructed region in the