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Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative
Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies
Authors
Tulpová, Z., Lu, M.C., Toegelová, H., Visendi, P., Hayashi, S., Vojta, P., Paux, E., Kilian, A., Abrouk, M., Bartoš, J., Hajdúch, M., Batley, J., Edwards, D., Doležel, D., Šimková, H.
Year
2019
Journal
New Biotechnology
Volume
48
Pages
12-19
DOI
10.1016/j.nbt.2018.03.003
Abstract
Bread
wheat
(Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding.
Physical
maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of
bread
wheat
and to assist
map
-based
gene
cloning
. As a part of a joint effort coordinated by the International
Wheat
Genome Sequencing Consortium, we have constructed a BAC-based
physical
map
of
bread
wheat
chromosome
arm
7DS
consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid
map
and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This
map
integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up
gene
cloning
in
wheat
. The process of
physical
map
assembly included the integration of the
7DS
physical
map
with a whole-genome
physical
map
of Aegilops tauschii and a
7DS
Bionano genome
map
, which together enabled efficient scaffolding of
physical
-
map
contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of
bread
wheat
and its ancestor at BAC-contig level and revealed a reconstructed region in the
7DS
pericentromere.