Genetic and developmental consequences of ploidy transitions in barley seeds

Category
PhD student
Group
Chromatin organization and function group

Description

Newly formed polyploids often show reduced seed viability, yet the developmental and genetic basis of this phenomenon remains poorly understood. This Ph.D. project will dissect how ploidy changes affect barley seed formation, with a strong focus on two ploidy-sensitive compartments—the embryo and endosperm—and on identifying critical developmental checkpoints disrupted by ploidy transitions. The student will help to establish an experimental framework enabling comparative analyses of seed development across diploid, triploid, and tetraploid backgrounds, including development of a triploid inducer line and generation of stable tetraploid barley lines. A central outcome will be a cytological and developmental atlas of seed formation based on existing and newly generated marker lines.

Keywords: Plants, seeds, polyploidy, microscopy, image analysis, barley (Hordeum vulgare)

Supervisor/Host
Dr. Anna Nowicka
is a senior scientist in the Plant Chromatin Organization and Function Group (headed by A. Pecinka) at the Center of Plant Structural and Functional Genomics, Institute of Experimental Botany in Olomouc, Czechia, specializing in chromatin organization and cereal seed development. She integrates cytogenetics, flow cytometry, advanced microscopy, and transcriptomics, and brings international postdoctoral experience (Poland and Germany) together with non-academic expertise in data analysis and visualization, with a strong interest in soft skills and public speaking.

Pecinka group website: olomouc.ueb.cas.cz/en/research-groups/pecinka-group
Bluesky (@pecinka-grp.bsky.social)
X (@pecinka_grp)

What you will learn& benefits 

  • Polyploid induction and ploidy verification by flow cytometry.
  • Genome editing (CRISPR/Cas) and generation of reporter lines.
  • Seed histology and developmental staging.
  • Advanced live-cell microscopy to visualize chromosome behavior, cellularization dynamics and cell-type-specific changes, including AI-assisted image analysis.
  • Professional skills: public speaking, scientific writing, data visualization, critical thinking and problem solving.
  • Competitivesalary according to IEB CAS rules; Social and health insurance, 25 days of vacation.
 

Who we are looking for & how to join us:

  • MSc (or equivalent) in plant biology/genetics/biotechnology
  • Send a CV and a brief motivation letter (PDF) to pecinkagroup@gmail.com (subject: Ph.D. Seed development).
  • The selection will start immediately and will continue until the position is filled.
  • The expected start is 1 September 2026.
  • Only the candidates considered for interview will be contacted.