The effects of the non-denaturing RGEN-ISL and standard denaturing FISH on the chromatin structure of maize were compared.
Comparison of the chromatin ultrastructure after Knob 2-specific labeling by RGEN-ISL (red) and subsequent FISH (green) of fixed chromosomes (A
) and interphase nuclei of maize (B
). To analyze the ultrastructure and spatial arrangement of signals and chromatin at a lateral resolution of ∼140 nm, 3D structured illumination microscopy was applied.