New publication: CRISPR/Cas9-Based RGEN-ISL allows the simultaneous and specific visualization of proteins, DNA repeats, and sites of DNA replication

Annotation: 
The effects of the non-denaturing RGEN-ISL and standard denaturing FISH on the chromatin structure of maize were compared. 
A, B Comparison of the chromatin ultrastructure after Knob 2-specific labeling by RGEN-ISL (red) and subsequent FISH (green) of fixed chromosomes (A) and interphase nuclei of maize (B). To analyze the ultrastructure and spatial arrangement of signals and chromatin at a lateral resolution of ∼140 nm, 3D structured illumination microscopy was applied.

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