The identification of genes of agronomic interest in bread wheat (Triticum aestivum
L.) is hampered by its allopolyploid nature and its considerable genome size, largely covered by transposable elements. In this study, the 2C gametocidal chromosome from Aegilops cylindrica
was used to develop a set of 113 deletion lines for chromosome 3D in the reference cultivar 'Chinese Spring'. Eighty-four markers were used to show that the deletions evenly covered chromosome 3D and ranged from 6.5 to 357 Mb. This new genetic stock will be useful for positional cloning of genes on chromosome 3D, especially for Ph2
affecting homoeologous pairing in bread wheat.