) gene encodes a MADS‐box transcription factor and plays an important role in the cold‐induced transition from the vegetative to reproductive stage. We performed an in‐depth sequence analysis of VRN1
homoeologs in a panel of 105 winter and spring varieties of hexaploid wheat. We described novel allele associated with reduced flowering time and intronic mutation which is transcribed into an alternative splice variant. Copy number variation analysis of VRN1
homoeologs showed that VRN‐B1
are present in only one copy in comparison to recessive vrn‐A1
, which ranged from one to four copies. Our results add another piece into the jigsaw puzzle called vernalization and could be used in the breeding of locally adapted cultivars.