Annotation:
Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. In this work we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues. We found that dividing nuclei have a round shape, adherent sister chromatids keep an equal amount of CENH3 histone and organize chromosomes according to a Rabl pattern. Endoreduplication alters the nuclear shape and causes positional loss of sister chromatid cohesion and loss of histone CENH3. In addition, they show a non-Rabl chromosome organization. We concluded that Rabl configuration is introduced and further reinforced by mitotic divisions in cell nuclei in a tissue- and seed age- dependent manner.
Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. In this work we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues. We found that dividing nuclei have a round shape, adherent sister chromatids keep an equal amount of CENH3 histone and organize chromosomes according to a Rabl pattern. Endoreduplication alters the nuclear shape and causes positional loss of sister chromatid cohesion and loss of histone CENH3. In addition, they show a non-Rabl chromosome organization. We concluded that Rabl configuration is introduced and further reinforced by mitotic divisions in cell nuclei in a tissue- and seed age- dependent manner.
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