RUVBL proteins are evolutionary conserved helicases which absence leads to complete lethality of the progeny. In this work we analyzed the role of RUVBLs in plant gametophyte development that could be the reason for absence of homozygous progeny. ruvbl
mutants have strongly impacted female gametogenesis producing an aberrant embryo sac. Additionally, male gametogenesis was compromised by reduced pollen germination and pollen tube guidance. Together, we conclude that RUVBL proteins are essential for proper gametophyte development.