Annotation:
„DNA-protein crosslinks, i.e. proteins covalently attached to DNA molecule represent extremely toxic type of DNA damage, which hinder the essential processes of replication and transcription. Here, we set-up forward-directed genetic screen to identify mutants hypersensitive to DNA protein crosslink inducing chemical zebularine that crosslinks METHYLTRANSFERASE1 (MET1) at 45S rDNA repeats. We mapped
HYPERSENSITIVE TO ZEBULARINE 1 (
HZE1) candidate as
SMC6B, a core component of Structural maintenance of chromosomes 5/6 (SMC5/6) complex. To shed light on the possible mechanism of SMC5/6 action, we focused on the Small ubiquitin modifier (SUMO) ligation activity of this complex, and showed that the accumulation of the DNA-protein crosslinks at the MET1-RFP foci induced by zebularine is SUMOylated in SMC5/6 complex dependent manner.“
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