Institute of Experimental
Botany of the AS CR, v.v.i.
About us
Research groups
Vacancies
Research
Projects
Publications
Technology
Flow cytometry
Reference DNA standards
Resources
Bananas
BarleyEpiBase
Cereals
Contact
Staff
en
cz
Home
Research
Publications
Development of deletion lines for chromosome 3D of bread wheat
Development of deletion lines for chromosome 3D of bread wheat
Authors
Svačina, R., Karafiátová, M., Malurová, M., Serra, H., Vítek, D., Endo, T.R., Sourdille, P., Bartoš J.
Year
2020
Journal
Frontiers in Plant Science
Volume
10
Pages
1756
DOI
10.3389/fpls.2019.01756
Abstract
The identification of genes of agronomic interest in bread wheat (
Triticum aestivum
L.) is hampered by its allopolyploid nature (2n = 6x = 42; AABBDD) and its very large genome, which is largely covered by transposable elements. However, owing to this complex structure, aneuploid stocks can be developed in which fragments or entire chromosomes are missing, sometimes resulting in visible phenotypes that help in the cloning of affected genes. In this study, the 2C gametocidal chromosome from
Aegilops cylindrica
was used to develop a set of 113 deletion lines for chromosome 3D in the reference cultivar Chinese Spring. Eighty-four markers were used to show that the deletions evenly covered chromosome 3D and ranged from 6.5 to 357 Mb. Cytogenetic analyses confirmed that the physical size of the deletions correlated well with the known molecular size deduced from the reference sequence. This new genetic stock will be useful for positional cloning of genes on chromosome 3D, especially for
Ph2
affecting homoeologous pairing in bread wheat.