Institute of Experimental
Botany of the AS CR, v.v.i.
Reference DNA standards
Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in
Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species
Himmelbach, A., Ruban, A., Walde, I., Šimková, H., Doležel, J., Hastie, A., Stein, N., Mascher, M.
occur in natural populations of many
, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion
are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by
(Hi-C) to detect
in four barley genotypes.
are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion
in natural populations.