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Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in
Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species
Authors
Himmelbach, A., Ruban, A., Walde, I., Šimková, H., Doležel, J., Hastie, A., Stein, N., Mascher, M.
Year
2018
Journal
Plant Journal
Volume
96
Pages
1309-1316
DOI
10.1111/tpj.14109
Abstract
Chromosomal
inversions
occur in natural populations of many
species
, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion
discovery
are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by
chromosome
-
conformation
capture
sequencing
(Hi-C) to detect
multi-megabase
polymorphic
inversions
in four barley genotypes.
Inversions
are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion
discovery
in natural populations.